Time-Resolved Fluorescence Wiki

start - [NovaFLIM / Analysis]

oschulz (oschulz@undisclosed.example.com) — 2026-02-11T14:19:03+00:00

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	{{topic>howto -FT300 -sample -analysis -draft &nodate&nouser}}		{{topic>howto -FT300 -sample -analysis -draft &nodate&nouser}}

-	===== NovaFLIM / Analysis =====
-
-	{{topic>howto +tutorial +novaflim -draft &nodate&nouser}}

	===== SymPhoTime64 / Analysis =====		===== SymPhoTime64 / Analysis =====

start - removed

oschulz (oschulz@undisclosed.example.com) — 2026-02-11T14:18:23+00:00

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-	{{tag> howto tutorial NovaFLIM}}
-

tutotial1 - removed

oschulz (oschulz@undisclosed.example.com) — 2026-02-11T14:18:00+00:00

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-	m a Batch Analysis Over Many Frames]( {{ :howto:novaflim:02_how_to_perform_a_batch_analysis_over_many_frames.pdf \| }}). {{ :howto:novaflim:02_how_to_perform_a_batch_analysis_over_many_frames.pdf \|}}	+

burst_duration_and_fcs_diffusion_time - [References]

doerr (doerr@undisclosed.example.com) — 2026-01-12T14:58:02+00:00

2026/01/12 15:56		current
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	Gopich, I. V.; Szabo, A. Journal of Chemical Physics 2005, 122, 014707.		Gopich, I. V.; Szabo, A. Journal of Chemical Physics 2005, 122, 014707.

-	Ruttinger, S. Dissertation, 2006.	+	Ruettinger, S. Dissertation, 2006.

-	Dorr, D. Dissertation, 2014.	+	Doerr, D. Dissertation, 2014.

applications

doerr (doerr@undisclosed.example.com) — 2026-01-12T10:25:53+00:00

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	[[howto:antibunching_measurements\|Photon antibunching measurements]]		[[howto:antibunching_measurements\|Photon antibunching measurements]]
		+
		+	[[howto:burst_duration_and_fcs_diffusion_time:burst_duration_and_fcs_diffusion_time\|Burst Duration and FCS Diffusion Time]]


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	[[howto:calculate_ratiometric_single_pair_fret_distributions\|Calculate ratiometric single pair FRET distributions]]		[[howto:calculate_ratiometric_single_pair_fret_distributions\|Calculate ratiometric single pair FRET distributions]]
		+
		+	[[howto:burst_duration_and_fcs_diffusion_time:burst_duration_and_fcs_diffusion_time\|Burst Duration and FCS Diffusion Time]]

howto

doerr (doerr@undisclosed.example.com) — 2026-01-12T10:03:28+00:00

2025/06/16 10:38		current
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	[[howto:phasor_plot_structure_separation:phasor_plot_structure_separation\|Phasor-Based Structural Separation Using FLIM]]		[[howto:phasor_plot_structure_separation:phasor_plot_structure_separation\|Phasor-Based Structural Separation Using FLIM]]
-

	[[howto:AX_FLIM_Examples:AX_FLIM_Examples\|NIS_Element_based_FLIM_Measurement_Examples]]		[[howto:AX_FLIM_Examples:AX_FLIM_Examples\|NIS_Element_based_FLIM_Measurement_Examples]]
		+
		+	[[howto:Burst Duration and FCS Diffusion Time:Burst Duration and FCS Diffusion Time\|Burst Duration and FCS Diffusion Time]]

ax_flim_examples

lan (lan@undisclosed.example.com) — 2025-06-16T08:53:39+00:00

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	1. How to automatically acquire both confocal and FLIM channels		1. How to automatically acquire both confocal and FLIM channels

-	{{ :howto:ax_flim_examples:nis-twochannel_publish.mp4 \|}}	+	{{ :howto:ax_flim_examples:nis-twochannel_publish.mp4?1000 \|}}

	2. How to perform FLIM stitching to visualize a large sample		2. How to perform FLIM stitching to visualize a large sample

-	{{ :howto:ax_flim_examples:nis_stiching_publish.mp4 \|}}	+	{{ :howto:ax_flim_examples:nis_stiching_publish.mp4?1000 \|}}

nis_stiching_publish.mp4 - created

lan (lan@undisclosed.example.com) — 2025-06-16T08:50:33+00:00

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nis-twochannel_publish.mp4 - created

lan (lan@undisclosed.example.com) — 2025-06-16T08:48:51+00:00

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phasor_plot_structure_separation

lan (lan@undisclosed.example.com) — 2025-06-16T08:36:34+00:00

2025/06/16 10:36		current
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	{{tag> FLIM acquisition Nikon AX Phasor video LSM_upgrade howto}}		{{tag> FLIM acquisition Nikon AX Phasor video LSM_upgrade howto}}

-	====== Phasor-Based Structural Separation Using FLIM ======	+	====== Nikon AX: Phasor-Based Structural Separation Using FLIM ======

	Introduction		Introduction

nis_phasor_publish.mp4 - created

lan (lan@undisclosed.example.com) — 2025-06-16T06:52:08+00:00

	current

01_how_to_select_rois_in_novaflim_based_on_parameter-histogram.pdf - created

lan (lan@undisclosed.example.com) — 2024-12-20T14:02:34+00:00

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02_how_to_perform_a_batch_analysis_over_many_frames.pdf - created

lan (lan@undisclosed.example.com) — 2024-12-19T16:11:00+00:00

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lifetime_component_decomposition_in_plasor_plot.mp4 - created

lan (lan@undisclosed.example.com) — 2024-09-13T14:53:10+00:00

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peter (peter@undisclosed.example.com) — 2022-08-04T13:11:52+00:00

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peter (peter@undisclosed.example.com) — 2022-08-04T13:08:13+00:00

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peter (peter@undisclosed.example.com) — 2022-08-03T14:42:34+00:00

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2025/06/16 10:38		current
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	[[howto:phasor_plot_structure_separation:phasor_plot_structure_separation\|Phasor-Based Structural Separation Using FLIM]]		[[howto:phasor_plot_structure_separation:phasor_plot_structure_separation\|Phasor-Based Structural Separation Using FLIM]]
-

	[[howto:AX_FLIM_Examples:AX_FLIM_Examples\|NIS_Element_based_FLIM_Measurement_Examples]]		[[howto:AX_FLIM_Examples:AX_FLIM_Examples\|NIS_Element_based_FLIM_Measurement_Examples]]
		+
		+	[[howto:Burst Duration and FCS Diffusion Time:Burst Duration and FCS Diffusion Time\|Burst Duration and FCS Diffusion Time]]

2025/06/16 10:53		current
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	1. How to automatically acquire both confocal and FLIM channels		1. How to automatically acquire both confocal and FLIM channels

-	{{ :howto:ax_flim_examples:nis-twochannel_publish.mp4 \|}}	+	{{ :howto:ax_flim_examples:nis-twochannel_publish.mp4?1000 \|}}

	2. How to perform FLIM stitching to visualize a large sample		2. How to perform FLIM stitching to visualize a large sample

-	{{ :howto:ax_flim_examples:nis_stiching_publish.mp4 \|}}	+	{{ :howto:ax_flim_examples:nis_stiching_publish.mp4?1000 \|}}