Time-Resolved Fluorescence Wiki

using_the_anisotropy_image_script

Anonymous (anonymous@undisclosed.example.com) — 2018-04-12T11:44:14+00:00

2018/04/12 12:26		current
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	Note:		Note:
-	The anisotropy values can theoretically spread between -0.5 and 1 for parallel or vertical excitation and emission dipoles respectively. The anisotropy value of 0 is expected in case of very fast rotation or extremely efficient energy transfer processes (e.g. efficient HOMO-FRET). When measuring a randomly aligned ensemble due to photoselection the anisotropy varies with in the limits of -0.2 and 0.4 instead of -0.5 and 1.	+	The anisotropy values can theoretically spread between -0.5 and 1 for perpendicular and parallel excitation and emission dipoles respectively. The anisotropy value of 0 is expected in case of very fast rotation or extremely efficient energy transfer processes (e.g. efficient HOMO-FRET). When measuring a randomly aligned ensemble due to photoselection the anisotropy varies with in the limits of -0.2 and 0.4 instead of -0.5 and 1.

	==== Adapt the script to the image and the system parameters ====		==== Adapt the script to the image and the system parameters ====

fluorescence_lifetime_measurements_using_the_fluotime_300

Anonymous (anonymous@undisclosed.example.com) — 2016-09-08T01:19:58+00:00

2016/09/08 03:18		current
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	{{ youtube>JLiDnlS5BS8?large }}		{{ youtube>JLiDnlS5BS8?large }}

-	This video demonstrates the special software application wizard of the FluoTime 300 system software for quantum yield measurements of Rhodamine 6G using an integrating sphere. The wizard guides the inexperienced user through all necessary steps to acquire high quality measurements.	+	This video demonstrates the special software application wizard of the FluoTime 300 system software for quantum yield measurements of Rhodamine 6G using an integrating sphere. The wizard guides the user through all necessary steps to acquire high quality measurements.

applications

Anonymous (anonymous@undisclosed.example.com) — 2026-01-12T10:25:53+00:00

2023/02/23 09:12		current
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	[[howto:antibunching_measurements\|Photon antibunching measurements]]		[[howto:antibunching_measurements\|Photon antibunching measurements]]
		+
		+	[[howto:burst_duration_and_fcs_diffusion_time:burst_duration_and_fcs_diffusion_time\|Burst Duration and FCS Diffusion Time]]


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	[[howto:calculate_ratiometric_single_pair_fret_distributions\|Calculate ratiometric single pair FRET distributions]]		[[howto:calculate_ratiometric_single_pair_fret_distributions\|Calculate ratiometric single pair FRET distributions]]
		+
		+	[[howto:burst_duration_and_fcs_diffusion_time:burst_duration_and_fcs_diffusion_time\|Burst Duration and FCS Diffusion Time]]

software

Anonymous (anonymous@undisclosed.example.com) — 2026-07-07T11:50:49+00:00

2025/05/28 09:47		current
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	*[[https://github.com/khemmen/katcorr/\|Katherinas correlator]] is a collection of scripts and correlators that are inspired by the paper Ries...Schwille 2010 Optics Express "Automated suppression of sample-related artifacts in FCS".		*[[https://github.com/khemmen/katcorr/\|Katherinas correlator]] is a collection of scripts and correlators that are inspired by the paper Ries...Schwille 2010 Optics Express "Automated suppression of sample-related artifacts in FCS".
	These scripts have different scopes and use the library [[https://github.com/Fluorescence-Tools/tttrlib\|tttrlib]] to read and correlate time-tagged time resolved (TTTR) files.		These scripts have different scopes and use the library [[https://github.com/Fluorescence-Tools/tttrlib\|tttrlib]] to read and correlate time-tagged time resolved (TTTR) files.
		+
		+	*[[https://github.com/yusuf-qutbuddin/theatRICS]] is a Python-based toolkit designed for the analysis of time-resolved fluorescence data. It is particularly well-suited for high-throughput data processing, offering efficient workflows for researchers managing large TCSPC datasets.
		+

	* [[http://flim-analyzer.ip-korea.org/\|FLIM-FRET analyzer]]: open source software for automation of lifetime-based FRET analysis. This is a Microsoft Windows application originally developed in C#. The main goal of this application is to provide the FLIM community a user-friendly application.		* [[http://flim-analyzer.ip-korea.org/\|FLIM-FRET analyzer]]: open source software for automation of lifetime-based FRET analysis. This is a Microsoft Windows application originally developed in C#. The main goal of this application is to provide the FLIM community a user-friendly application.

fluofit

Anonymous (anonymous@undisclosed.example.com) — 2015-11-03T14:07:12+00:00

2015/11/03 15:07		current
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	====== FluoFit ======		====== FluoFit ======
-	The FluoFit software package is a powerful global analysis software for fluorescence decay and anisotropy measurements. Tail fitting as well as a numerical reconvolution algorithm to account for the finite Instrument Response Function ([[IRF]]) can be applied. The FluoFit software is available in two versions (Basic and Professional) with different functionalities to meet individual needs.	+	The FluoFit software package is a powerful global analysis software for fluorescence decay and anisotropy measurements. Tail fitting as well as a numerical reconvolution algorithm to account for the finite Instrument Response Function ([[glossary:IRF]]) can be applied. The FluoFit software is available in two versions (Basic and Professional) with different functionalities to meet individual needs.

	see [[https://www.picoquant.com/products/category/software/fluofit-global-fluorescence-decay-data-analysis-software]]		see [[https://www.picoquant.com/products/category/software/fluofit-global-fluorescence-decay-data-analysis-software]]

measurement_hardware_instrumentation

Anonymous (anonymous@undisclosed.example.com) — 2018-06-25T07:18:07+00:00

2018/06/25 09:17		current
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	[[video_tutorials#Kurt Thorn: Optical Sectioning and Confocal Microscopy\|Confocal microscoy and optical sectioning by Kurt Thorn]]		[[video_tutorials#Kurt Thorn: Optical Sectioning and Confocal Microscopy\|Confocal microscoy and optical sectioning by Kurt Thorn]]

-	[[technical docs:Beampath of the Zeiss LSM880]]	+	[[Beampath of the Zeiss LSM880]]

	[[howto:check_overlap_of_different_color_confocal_volumes\|How to check the overlap of different color confocal volumes]]		[[howto:check_overlap_of_different_color_confocal_volumes\|How to check the overlap of different color confocal volumes]]

start

Anonymous (anonymous@undisclosed.example.com) — 2021-06-17T14:47:30+00:00

2020/05/04 14:11		current
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-	<WRAP center round box 60%>
-	===== News from the community=====
-	* 29.04.20 [[https://www.fret.community/announcement-protein-folding-and-dynamics-webinar/\|Protein folding and dynamics Webinar-Series.]]
-	* 14.04.20 [[https://analyticalscience.wiley.com/do/10.1002/was.00050003/full/bioimagedataanalysis.pdf\|Bioimage Data Analysis.]] New Expanded version of a nice open-source introduction. \\ \\
-	* 14.04.20 [[https://science.sciencemag.org/content/368/6486/78\|De novo design of protein logic gates.]] Exciting fundamental enzymology work. Chen et al. describe the design of logic gates that can regulate protein association. \\ \\
-
-
-
-	[[general:community_news\|...see more...]]
-	</WRAP>

2025/05/28 09:47		current
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	*[[https://github.com/khemmen/katcorr/\|Katherinas correlator]] is a collection of scripts and correlators that are inspired by the paper Ries...Schwille 2010 Optics Express "Automated suppression of sample-related artifacts in FCS".		*[[https://github.com/khemmen/katcorr/\|Katherinas correlator]] is a collection of scripts and correlators that are inspired by the paper Ries...Schwille 2010 Optics Express "Automated suppression of sample-related artifacts in FCS".
	These scripts have different scopes and use the library [[https://github.com/Fluorescence-Tools/tttrlib\|tttrlib]] to read and correlate time-tagged time resolved (TTTR) files.		These scripts have different scopes and use the library [[https://github.com/Fluorescence-Tools/tttrlib\|tttrlib]] to read and correlate time-tagged time resolved (TTTR) files.
		+
		+	*[[https://github.com/yusuf-qutbuddin/theatRICS]] is a Python-based toolkit designed for the analysis of time-resolved fluorescence data. It is particularly well-suited for high-throughput data processing, offering efficient workflows for researchers managing large TCSPC datasets.
		+

	* [[http://flim-analyzer.ip-korea.org/\|FLIM-FRET analyzer]]: open source software for automation of lifetime-based FRET analysis. This is a Microsoft Windows application originally developed in C#. The main goal of this application is to provide the FLIM community a user-friendly application.		* [[http://flim-analyzer.ip-korea.org/\|FLIM-FRET analyzer]]: open source software for automation of lifetime-based FRET analysis. This is a Microsoft Windows application originally developed in C#. The main goal of this application is to provide the FLIM community a user-friendly application.