Time-Resolved Fluorescence Wiki

old_dongles_drivers_for_fluofit_symphotime_32

Anonymous (anonymous@undisclosed.example.com) — 2025-03-14T10:04:27+00:00

2025/03/14 11:02		current
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	Our team successfully tested this setup using VMware Workstation Player 15.5.1, where both FluoFit and SPT32 dongles worked on an emulated Windows 7 (x64). Consider the following points:		Our team successfully tested this setup using VMware Workstation Player 15.5.1, where both FluoFit and SPT32 dongles worked on an emulated Windows 7 (x64). Consider the following points:

-	You will need valid licenses for both Windows 10/8 (host system) and Windows 7 (guest system).	+	You will need valid licenses for both newer Windows version (host system) and Windows 7 (guest system).

	VMware Workstation Player is free for non-commercial use, but the VM setup must be done by the user.		VMware Workstation Player is free for non-commercial use, but the VM setup must be done by the user.

Anonymous (anonymous@undisclosed.example.com) — 2025-02-14T09:43:52+00:00

2025/02/14 10:43		current
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	In this article we assume that both detectors are SPADs of the same kind (both Excelitas SPADs, or both PDM-SPADs, or both tau-SPADs).		In this article we assume that both detectors are SPADs of the same kind (both Excelitas SPADs, or both PDM-SPADs, or both tau-SPADs).

-	If you are using either a proper (see below) 50/50 cube beamsplitter to split the signal between two detectors or a 100% mirror to direct the signal to one detector and then the other, but still observe very different count rates on the two detectors, this discrepancy could be due to the following reasons:	+	If you are using either a proper (see below) 50/50 beamsplitter to split the signal between two detectors or a 100% mirror to direct the signal to one detector and then the other, but still observe very different count rates on the two detectors, this discrepancy could be due to the following reasons:

	1. Misalignment of the Lens in Front of the SPAD:		1. Misalignment of the Lens in Front of the SPAD:

Anonymous (anonymous@undisclosed.example.com) — 2025-03-11T08:23:49+00:00

2025/03/11 09:23		current
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	{{tag>FAQ LDH PLS PDL Laser SymPhoTime SPT SEPIA}}		{{tag>FAQ LDH PLS PDL Laser SymPhoTime SPT SEPIA}}

-	====== The intensity response of my laser is not linear ======	+	====== The Intensity Response of my Laser is not linear ======
	or		or
-	====== My laser does not turn on with low power ======	+	====== My Laser does not turn on with low Power ======

	===== Background =====		===== Background =====

Anonymous (anonymous@undisclosed.example.com) — 2025-02-14T15:46:14+00:00

2025/01/31 09:57		current
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	====== The Dark Counts of my SPADs increased. When do I need to take action? ======		====== The Dark Counts of my SPADs increased. When do I need to take action? ======
-	{{tag> detector faq SPAD dcps dark_counts}}	+	{{tag> detectors faq SPAD dcps dark_counts}}

	SPAD detectors (Excelitas SPCMs or MPD PDMs) are relatively robust devices, but they can exhibit ageing, which shows mostly in the level of dark counts. Dark counts are the count rate on the detector when no light at all can get to the active area of the detector.		SPAD detectors (Excelitas SPCMs or MPD PDMs) are relatively robust devices, but they can exhibit ageing, which shows mostly in the level of dark counts. Dark counts are the count rate on the detector when no light at all can get to the active area of the detector.

Anonymous (anonymous@undisclosed.example.com) — 2025-02-14T15:49:47+00:00

2025/01/31 10:01		current
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	====== How can I analyze my data using non-PicoQuant software? ======		====== How can I analyze my data using non-PicoQuant software? ======

-	{{tag> faq file_format ptu open_source analysis}}	+	{{tag> faq file_format ptu open_source analysis software}}

	The PicoQuant raw-data format is freely accessible. PicoQuant has published some demo scripts for reading the different TCSPC/TTTR data formats on Github:		The PicoQuant raw-data format is freely accessible. PicoQuant has published some demo scripts for reading the different TCSPC/TTTR data formats on Github:

Anonymous (anonymous@undisclosed.example.com) — 2025-02-14T15:50:52+00:00

2025/02/14 16:40		current
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	====== NovaFLIM Licenses ======		====== NovaFLIM Licenses ======
		+	{{tag> faq software NovAFLIM license}}
		+

	For NovaFLIM, we use a software license instead of a hardware dongle to make license management more efficient. Compared to a hardware dongle, this approach eliminates the time and costs associated with handling and shipping dongles, as well as the risk of losing or removing them.		For NovaFLIM, we use a software license instead of a hardware dongle to make license management more efficient. Compared to a hardware dongle, this approach eliminates the time and costs associated with handling and shipping dongles, as well as the risk of losing or removing them.

Anonymous (anonymous@undisclosed.example.com) — 2025-02-14T15:47:18+00:00

2025/02/14 16:45		current
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	====== Why do I not get the same count rates on both SPAD detectors? ======		====== Why do I not get the same count rates on both SPAD detectors? ======
-	{{tag> detectors faq SPAD}}	+	{{tag> detectors faq SPAD MT200}}

	In this article we assume that both detectors are SPADs of the same kind (both Excelitas SPADs, or both PDM-SPADs, or both tau-SPADs).		In this article we assume that both detectors are SPADs of the same kind (both Excelitas SPADs, or both PDM-SPADs, or both tau-SPADs).

Anonymous (anonymous@undisclosed.example.com) — 2025-03-11T08:19:29+00:00

2025/02/18 14:49		current
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	====== NovaFLIM Calculated IRF ======		====== NovaFLIM Calculated IRF ======
		+	{{tag> faq software novaflim IRF}}
		+

	In the Nova Flim the IRF is parameterized as a combination of two Gaussian curves (a total of 5 fit parameters). These parameters are adjusted during Initial Fit.		In the Nova Flim the IRF is parameterized as a combination of two Gaussian curves (a total of 5 fit parameters). These parameters are adjusted during Initial Fit.