https://tcspc.com/ 2026-04-23T18:51:43+00:00 https://tcspc.com/ https://tcspc.com/lib/exe/fetch.php/logo.png text/html 2018-04-12T11:44:14+00:00 Anonymous (anonymous@undisclosed.example.com) https://tcspc.com/doku.php/howto:using_the_anisotropy_image_script?rev=1523533454&do=diff <table><tr><th colspan="2" width="50%">2018/04/12 12:26</th><th colspan="2" width="50%">current</th></tr><tr><td class="diff-blockheader" colspan="2">Line 52:</td> <td class="diff-blockheader" colspan="2">Line 52:</td> </tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context"></td><td class="diff-lineheader">&#160;</td><td class="diff-context"></td></tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context">**Note:**</td><td class="diff-lineheader">&#160;</td><td class="diff-context">**Note:**</td></tr> <tr><td class="diff-lineheader">-</td><td class="diff-deletedline">&#160;The anisotropy values can theoretically spread between -0.5 and 1 for parallel&#160;<strong class="diff-mark">or vertical&#160;</strong>excitation and emission dipoles respectively. The anisotropy value of 0 is expected in case of very fast rotation or extremely efficient energy transfer processes&#160; (e.g. efficient&#160; HOMO-FRET).&#160; When measuring a randomly aligned ensemble due to photoselection the anisotropy varies with in the limits of&#160; -0.2 and 0.4 instead of -0.5 and 1.&#160; &#160; &#160;&#160;</td><td class="diff-lineheader">+</td><td class="diff-addedline">&#160;The anisotropy values can theoretically spread between -0.5 and 1 for&#160;<strong class="diff-mark">perpendicular and&#160;</strong>parallel excitation and emission dipoles respectively. The anisotropy value of 0 is expected in case of very fast rotation or extremely efficient energy transfer processes&#160; (e.g. efficient&#160; HOMO-FRET).&#160; When measuring a randomly aligned ensemble due to photoselection the anisotropy varies with in the limits of&#160; -0.2 and 0.4 instead of -0.5 and 1.&#160; &#160; &#160;&#160;</td></tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context"></td><td class="diff-lineheader">&#160;</td><td class="diff-context"></td></tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context">==== Adapt the script to the image and the system parameters ====</td><td class="diff-lineheader">&#160;</td><td class="diff-context">==== Adapt the script to the image and the system parameters ====</td></tr> </table>