This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case a Zeiss LSM710. In FLIM-measurements, the fluorescence lifetime of a component can be determined which can be used e.g.

• to distinguish different tissue or cell constituents

• to monitor concentration changes using a reporter dye

• To monitor interactions between two molecules via FRET.
  

The data acquisition is analogous also for upgrades on an LSM780 or LSM880, and the general principles of the data acquisition are analogous in all LSM upgrade kits.

Continue to

• How to measure the Instrument Response function (IRF)