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advantages_and_disadvantages_of_two_photon_excitation_tpe @general
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servation time of 12.5 ns in the life time of the dyes. This is too short for most of the dyes. With pulse pickers the repetition rate can be re
separation_of_2_species_with @howto
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nstant is too similar to distinguish between both dyes in a mixture by means of standard FCS.\\ Nevertheless, the autocorrelation curves of both dyes differ in the microsecond range. ATTO655 shows pr... somerization.\\ The fluorescence lifetime of both dyes differ significantly (~1.8 ns for ATTO655 and ~1 ... s to define these filters: * If the different dyes have been measured separately, and TCPSC curves o
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
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ions between two molecules labeled with different dyes. Usually, the dyes attached to the two molecules differ in their abs... abeled with both fluorophores.\\ FCCS calibration dyes are available e.g. from IBA ([[http://www.iba-lif... ally, a positive control is needed, in which both dyes are moving in a coordinated fashion.\\ Additional
calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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* **Note** that the diffusion coefficients of all dyes are temperature dependent. * FCS measurements a... e through the confocal volume. Examples for these dyes are ATTO655 or ATTO425. Also dyes with a moderate triplet contribution can be fitted with this model if... cell membrane is observed. This model is made for dyes moving within a plane, e.g. modified lipids in a
calculate_fccs_trace_with_the_grouped_fcs_script @howto
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ions between two molecules labeled with different dyes. Usually, the dyes attached to the two molecules of interest differ ... abeled with both fluorophores.\\ FCCS calibration dyes are available e.g. from IBA ([[http://www.iba-lif
fluorescence_correlation_spectroscopy-_a_short_introduction @general
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/7353/appnote_diffusioncoefficients.pdf| standard dyes for FCS]] and perform a series of measurements wh... Diffusion Coefficients of Organosoluble Reference Dyes in Organic Media Measured by Dual-Focus Fluoresce
some_origins_of_multiexponetial_decays_for_single_dyes
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= Some origins of multiexponetial decays for pure dyes ====== The fluorescence lifetime of a dye measu... c angle in a perfectly aligned spectrometer pure dyes in homogeneous media may exhibit a multiexponenti
measurement_hardware_instrumentation
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Some origins of multiexponetial decays for single dyes|Some origins of multiexponetial decays for pure dyes]] ===== Microscopy ===== [[http://www.picoquan
lifetime_fitting_using_the_tcpsc_fitting_script @howto
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c_fitting_script_Image_17.png?600 }} * If both dyes can be fitted with the same model, click "Fit All... le, it is of course meaningless, as two different dyes were fitted. * Place the mouse over the graph,
how_to_measure_the_instrument_response_function_irf @howto
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/10.1088/0957-0233/27/2/027001 Pyridinium Styryl Dyes have also been used as IRF standard: Lei Li, Men... lph Jimenez, Jianhua Xu\\ Using Pyridinium Styryl Dyes as the Standards of Time-Resolved Instrument Resp
calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
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o distinguish molecules with and without acceptor dyes and to filter the events for signals without acce
pattern_matching @howto
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on raw data. This allows for separating different dyes or regions of different lifetime/decay-behavior i
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
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te.\\ Note that the diffusion coefficients of all dyes are temperature dependent.\\ FCS measurements are
flim_fret_calculation_for_multi_exponential_donors @howto
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TCSPC-window, e.g. 2PE excitation with 80MHz and dyes with a lifetime above ~3ns. For explanation on th