Search
You can find the results of your search below.
Matching pagenames:
- flim_fcs_using_olympus_fluoview_fv3000_lsm_upgrade_kit
- fcs (glossary)
- 2ffcs (howto)
- calculate_and_fit_fcs_traces_with_the_fcs_script
- calculate_fccs_trace_with_the_grouped_fcs_script
- calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script
- flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade
- flim-fret_measurement_using_an_olympus_fv1200_with_a_flim_and_fcs_upgrade
- mt200fcs (howto)
- performing_an_fcs_measurement_with_an_olympus_fv1200_upgrade_kit
- fcs_viewer (software)
- burst_duration_and_fcs_diffusion_time
Fulltext results:
- calculate_and_fit_fcs_traces_with_the_fcs_script @howto
- ymPhoTime}} ~~NOTOC~~ ====== Calculate and Fit FCS Traces with the FCS Script ====== ===== Summary ===== This tutoria... ft side of the main window. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_1.png }} * Highlight the file ''A... '' by a single mouse click. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_2.png }} * Select the "Analysis"-... en the dropdown menu "FCS". {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_3.png }} **Note:** The drop-down me
- calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
- measurements with a calibration dye. Therefore, a FCS curve is calculated with the FCS script, that is transferred to the FCS Calibration script. {{ youtube>K_dyVx-l_tY?large... d|SPAD]] or [[glossary:Hybrid PMT]] detectors).\\ FCS is a technique applicable over a wide range of concentrations, but ideal FCS concentrations are usually between 1 and 20 nM. =... ain window. {{ calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script_Image_1.png }} * Highlight ... ouse click. {{ calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script_Image_2.png }} * Select the
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- FCS measurements.]] * [[howto:Calculate and Fit FCS Traces with the FCS Script| Calculation and fitting FCS curves with the SymPhoTIme64 software]] * [[howto:calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script| Calibration of the confocal v... serve as examples. There are many more excellent FCS-related articles that could guide you through the use of FCS. General Introduction to the theory, the techni... tudies in cell membranes" * **Lifetime-filtered FCS** \\ Ishii et al., 2015, "Lifetime-Weighted FCS and 2D FLCS:Advanced Application of Time-Tagged T
- calculate_fccs_trace_with_the_grouped_fcs_script @howto
- d in detail in the tutorial: "[[calculate_and_fit_fcs_traces_with_the_fcs_script|Calculate and Fit FCS Traces with the FCS Script]]". Look there to find out, how to proceed. ==== Need for calibration to r
- separation_of_2_species_with @howto
- ected in this graph. - **Lower center/right:** FCS trace window. As first the FCS correlation criteria have to be defined and the c... ng "Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:Calculate and Fit FCS Traces with the FCS Script]]. ==== Definition of... be fitted by selecting "Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:Calculate and Fit FCS Traces with the FCS Script]].
- applications
- s FV1200 upgrade kit]] [[howto:calculate_and_fit_fcs_traces_with_the_fcs_script|Calculate and fit FCS curves]] [[howto:calibrate_the_confocal_volume_f... nching measurements]] [[howto:burst_duration_and_fcs_diffusion_time:burst_duration_and_fcs_diffusion_time|Burst Duration and FCS Diffusion Time]] ==== Single Molecule Detection... FRET distributions]] [[howto:burst_duration_and_fcs_diffusion_time:burst_duration_and_fcs_diffusion_time|Burst Duration and FCS Diffusion Time]] ==== Anisotropy ==== [[howto:
- software
- sed Pycorrfit*/ * [[https://github.com/dwaithe/FCS_point_correlator| FCS Point Correlator]] FCS Bulk Correlation Software accepting pt3 and ptu f... e.github.io/FCSfitJS/ [[https://dwaithe.github.io/FCSfitJS/| FCS fit JS]] * [[software:FLIMFit]]. FLIMfit is an... ata visualisation. [[https://github.com/dwaithe/FCS_point_correlator | FoCuS Homepage]] and [[http:/
- burst_duration_and_fcs_diffusion_time @howto:burst_duration_and_fcs_diffusion_time
- al howto analysis calibration PSF confocal-volume FCS burst single molecules measurement SMD Diffusion... duration}} ~~NOTOC~~ ====== Burst duration and FCS diffusion time ====== **Diffusion, flow, burst se... acted from fluorescence correlation spectroscopy (FCS) and the burst duration obtained from single-mole... -dimensional Gaussian detection model ===== Both FCS and burst-based single-molecule experiments rely
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
- tified in this graph. - **Lower center/right:** FCS trace. As first the FCS correlation criteria have to be defined and the c... al FIT" to fit these patterns to the decay of the FCS measurement. {{ using_the_flcs_script_for_spectr... nlinked_mix.ptu'') is active. ==== Calculate the FCS crosscorrelation curve ==== * Press "Cross Cor... f the FCCS correlation obtained with the "Grouped FCS" script to better see the effect. {{ using_the_fl
- symphotime_tips_and_tricks @howto
- the script with the stored result - For online FCS-results, the calculated molecular brightness can ... result-file opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on ... me]], the image consists of a single frame) === FCS (for each of the max.2 data channels) === <WRAP> <imgcaption CommentFCS| Comment Tab of an FCS online analysis result>{{onlinefcs-3.png?300|}}</
- pycorrfit @software
- {{tag> software analysis open_source FCS}} ====== PyCorrFit: data analysis software for fluorescence correlation spectroscopy (FCS) ====== "[...] PyCorrFit is a general-purpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-i
- 2ffcs @howto
- fusion length into the analysis. see [[glossary:FCS]] for a introduction on FCS. ===== Experimental Setup ===== ==== Pinhole Sel... i === The distance between the foci in two-focus FCS is determined by the Nomarski prism. Usually the
- performing_an_fcs_measurement_with_an_olympus_fv1200_upgrade_kit @howto
- {{tag> howto FCS acquisition video FV1200 Olympus LSM_upgrade}} ====== Performing a FCS measurement with an Olympus FV1200 Upgrade Kit===... } This video is a walkthrough of how to measure a FCS (Fluorescence Correlation Spectroscopy) trace with an Olympus FV1200 upgraded for FLIM and FCS with a PicoQuant upgrade kit. Continue to: {{t
- howto @howto
- ). ==== General ==== {{topic>tutorial +analysis -FCS -FRET -FLIM -draft &nodate&nouser}} ==== FLIM ==== {{topic>tutorial +analysis +FLIM -FCS -FRET -draft &nodate&nouser}} ==== FRET ==== {{t... al +analysis +FRET -draft &nodate&nouser}} ==== FCS / Correlation ==== {{topic>tutorial +analysis +FCS -draft &nodate&nouser}} ===== EasyTau2 / Analysis
- fcs_viewer @software
- {{tag>software analysis FCS open_source}} ====== FCS viewer: FCS data visualizer for PicoQuant data format ====== [[https://github.com/paulmueller/FCS_viewer]] Contains a port of the PicoQuant Matlab