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calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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{{tag> tutorial howto FCS analysis SymPhoTime}} ~~NOTOC~~ ====== Calculate and Fit FCS Traces with the FCS Script ====== ===== Summary ===== This tutorial shows step-by-step, how an auto... olved in water is calculated and fitted using the FCS script. {{ youtube>NRFaEuSb778?large }} ===== B
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
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wto analysis calibration PSF confocal-volume VEFF FCS SymPhoTime}} ~~NOTOC~~ ====== Calibrate the Confocal Volume for and with FCS ====== ===== Summary ===== This tutorial shows... by-step, how to calibrate the confocal volume for FCS measurements with a calibration dye. Therefore, a FCS curve is calculated with the FCS script, that is
fluorescence_correlation_spectroscopy-_a_short_introduction @general
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{{tag>MicroTime LSM Theory SymPhoTime FCS Correlation}} ====== Introduction to Fluorescence Correlat... und ===== Fluorescence Correlation Spectroscopy (FCS) is founded on the analysis of the fluctuations o... rescence intensity detected from a given system. FCS was introduced in the 1970's by Magde, Elson and ... ules. In short time, the theoritical framework of FCS was expanded to incorporate also the study of rot
calculate_fccs_trace_with_the_grouped_fcs_script @howto
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{{tag> analysis howto tutorial FCS FCCS Cross-Correlation SymPhoTime}} ~~NOTOC~~ ====== Calculate FCCS Traces with the Grouped FCS Script ====== ===== Summary ===== This tutoria... dye-labeled DNA-oligonucleotide using the Grouped FCS script. {{ youtube>0Ht_L3a53E0?large }} ===== B... ngle mouse click. **Note:** This file contains a FCS measurement of a FCCS positive control, a DNA oli
separation_of_2_species_with @howto
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{{tag> howto tutorial FCS FLCS analysis SymPhoTime}} ~~NOTOC~~ ====== Separation of 2 Species with Di... tween both dyes in a mixture by means of standard FCS.\\ Nevertheless, the autocorrelation curves of bo... tically no photophysical fluctuations. Therefore, FCS curves of ATTO655 can be fitted with the simplest... ontrary, exhibits a distinct bunching term on its FCS trace originating from photophysical processes, i
software
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ww.mpc.hhu.de/software/3-software-package-for-mfd-fcs-and-mfis|Software Package for Multiparameter Fluo... * [[software:PyCorrFit]] is a general-purpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-i... a wide range of applications in standard confocal FCS. In addition, it contains equations dealing with
applications
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]] ==== Fluorescence Correlation Spectroscopy (FCS) ==== [[general:fluorescence_correlation_spectro... py-_a_short_introduction| A short introduction to FCS with suggestions for further reading]] [[howto:Performing an FCS Measurement with an Olympus FV1200 upgrade kit]] [[howto:calculate_and_fit_fcs_traces_with_the_fcs_script|Calculate and fit FCS
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
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{{tag> howto tutorial FCS FLCS crosstalk analysis SymPhoTime}} ~~NOTOC~~ ====== Spectral Crosstalk Rem... tutorial [[Calculate FCCS Trace with the Grouped FCS Script]]. **Note:** This way you can compare the... ngle mouse click. **Note:** This file contains a FCS measurement of a FCCS negative control (two DNA o... lysis" tab and in there, open the drop down menu "FCS". {{ using_the_flcs_script_for_spectral_crosstalk
symphotime_tips_and_tricks @howto
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the script with the stored result - For online FCS-results, the calculated molecular brightness can ... result-file opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on ... me]], the image consists of a single frame) === FCS (for each of the max.2 data channels) === <WRAP> <imgcaption CommentFCS| Comment Tab of an FCS online analysis result>{{onlinefcs-3.png?300|}}</
2ffcs @howto
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{{tag> 2fFCS FCS}} ~~TOC~~ ====== 2fFCS ====== 2fFCS is an abbreviation **2 F**ocus **F**luorescence **C**orrelation **S**pectroscopy. Dual focus FCS might also be used. Correlation analysis that is... fusion length into the analysis. see [[glossary:FCS]] for a introduction on FCS. ===== Experimental Setup ===== ==== Pinhole Selection ==== The conjugat
performing_an_fcs_measurement_with_an_olympus_fv1200_upgrade_kit @howto
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{{tag> howto FCS acquisition video FV1200 Olympus LSM_upgrade}} ====== Performing a FCS measurement with an Olympus FV1200 Upgrade Kit===... } This video is a walkthrough of how to measure a FCS (Fluorescence Correlation Spectroscopy) trace with an Olympus FV1200 upgraded for FLIM and FCS with a PicoQuant upgrade kit. Continue to: {{t
pycorrfit @software
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{{tag> software analysis open_source FCS}} ====== PyCorrFit: data analysis software for fluorescence correlation spectroscopy (FCS) ====== "[...] PyCorrFit is a general-purpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-i
measurement_hardware_instrumentation
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or Confocal Time-resolved Microscopy (FLIM, FRET, FCS)]] - Poster (pdf format) [[video_tutorials|Fluo... FLIM Measurement using a Nikon A1 with a FLIM and FCS upgrade]] [[howto:Recording a Fluorescence Lifet... asurement using an Olympus FV1200 with a FLIM and FCS upgrade]] [[howto:Performing an FCS measurement with an Olympus FV1200 upgrade kit]] [[howto:exchang
howto @howto
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). ==== General ==== {{topic>tutorial +analysis -FCS -FRET -FLIM -draft &nodate&nouser}} ==== FLIM ==== {{topic>tutorial +analysis +FLIM -FCS -FRET -draft &nodate&nouser}} ==== FRET ==== {{t... al +analysis +FRET -draft &nodate&nouser}} ==== FCS / Correlation ==== {{topic>tutorial +analysis +FCS -draft &nodate&nouser}} ===== EasyTau2 / Analysis
fcs_viewer @software
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{{tag>software analysis FCS open_source}} ====== FCS viewer: FCS data visualizer for PicoQuant data format ====== [[https://github.com/paulmueller/FCS_viewer]] Contains a port of the PicoQuant Matlab
symphotime64 @software
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flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade @howto
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advantages_and_disadvantages_of_two_photon_excitation_tpe @general
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fcs @glossary
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mt200fcs @howto
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phasor_analysis @howto
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microtime @products
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basics
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lifetime_component_decomposition_phasor_plot_analysis
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lsm710
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start
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community_news @general
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t2-mode @glossary
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antibunching_measurements @howto
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calculate_ratiometric_single_pair_fret_distributions @howto
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calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
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determination_of_the_focal_width_with_the_focal @howto
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flim-fret_measurement_using_an_olympus_fv1200_with_a_flim_and_fcs_upgrade @howto
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intensity_time_trace_analysis @howto
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using_the_antibunching_script @howto
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hydraharp_400 @products
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picoharp_300 @products
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supported_mt200_pc_configuration_for_symphotime_64 @software
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