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- flim_fret_calculation_for_multi_exponential_donors @howto
- adapted.\\ **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... hen applied to the lifetime graph(s) in the TCSPC histogram.\\ **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
- flim-fret_calculation_for_single_exponential_donors @howto
- . - **Upper right: Fluorescence FRET Efficiency Histogram.** As the FRET efficiency image has not been calc... the right of the plot. - **Lower left: Binding histogram.** As the FRET efficiency image has not been calculated yet, this histogram is still empty. This diagram can only be calculat... al decay kinetics. - **Lower center/left: TCSPC histogram of all photons in the image.** In green (= datase
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- orial shows step-by-step, how to calculate a FRET histogram from single [[glossary:fret|FRET]]pairs detected ... s still empty.\\ - **Right:** FRET distribution histogram and the FRET radius histogram. As these distributions have not been calculated yet, this area is still empty... s region is used to calculate the FRET efficiency histogram. The second area shows the fluorescence response
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- lifetime-fitting_using_the_flim_script @howto
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- lifetime_fitting_using_the_flim_analysis @howto
- ted.\\ **Upper right:** the Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... the lifetime graph(s) on the lower center (TCSPC histogram).\\ **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
- update @howto
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- roi_fitting_using_the_flim_script @howto
- ted.\\ - **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... the lifetime graph(s) on the lower center (TCSPC histogram).\\ - **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
- ted.\\ - **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... o the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\ - **Lower center/left:** [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red a
- calculate_ratiometric_fret-images @howto
- \\ - **Lower center:** Here the FRET efficiency histogram over the selected pixels will be plotted when the... ed.\\ - **Lower right:** Here the FRET distance histogram in terms of R0 will be plotted when the calculati... o 100% FRET efficiency. * The FRET efficiency histogram and the Distances histogram are shown. {{ calculate_ratiometric_fret-images_with_the_fret-image-script_Image
- calculate_ratiometric_single_pair_fret_distributions @howto
- y-step, how to calculate a [[glossary:fret|FRET]] histogram from single FRET pairs detected under single mole... .\\ - **Lower center/right:** FRET distribution histogram and FRET radius histogram. As these distributions have not been calculated yet, this area is still empty. ... s region is used to calculate the FRET efficiency histogram. The unmarked white area shows the fluorescence r
- separation_of_2_species_with @howto
- e above highlighted in green. The photon counting histogram on the right displays the frequencies of the diff... ons, a lifetime fit can be performed of the TCPSC histogram of the measurement. The FLCS filters can be calcu... different parameters. - **Upper center:** TCSPC histogram of the imported patterns. As we haven't loaded an... mage_11.png?384 }} **Response:** * The TCSPC histogram for this file appears in the "Patterns" graph in
- using_the_anisotropy_image_script @howto
- olecules to calculate the static anisotropy and a histogram with the anisotropy distribution. {{ youtube>Tyc... w opens which contains the anisotropy image and a histogram with the anisotropy distribution over the differe... tween -0.4 and 1. - **Lower graph:** Anisotropy histogram over the different pixels. **Note:** The anisot... the display settings for the anisotropy frequency histogram in the corresponding controls. {{ using_the_aniso
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- er/right:** TCPSC fitting graph showing the TCSPC histogram of the measured sample. * In the decay form o... PC histograms of both files are loaded. The TCSPC histogram from the file "ATTO655_diff_FLCS-pattern.ptu is m... measurement of the dye Cy5 in water. In the TCSPC histogram it can be clearly seen that the lifetime of this