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- flim_fret_calculation_for_multi_exponential_donors @howto
- adapted.\\ **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... hen applied to the lifetime graph(s) in the TCSPC histogram.\\ **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- orial shows step-by-step, how to calculate a FRET histogram from single [[glossary:fret|FRET]]pairs detected ... s still empty.\\ - **Right:** FRET distribution histogram and the FRET radius histogram. As these distributions have not been calculated yet, this area is still empty... s region is used to calculate the FRET efficiency histogram. The second area shows the fluorescence response
- flim-fret_calculation_for_single_exponential_donors @howto
- . - **Upper right: Fluorescence FRET Efficiency Histogram.** As the FRET efficiency image has not been calc... the right of the plot. - **Lower left: Binding histogram.** As the FRET efficiency image has not been calculated yet, this histogram is still empty. This diagram can only be calculat... al decay kinetics. - **Lower center/left: TCSPC histogram of all photons in the image.** In green (= datase
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- lifetime-fitting_using_the_flim_script @howto
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- calculate_ratiometric_fret-images @howto
- \\ - **Lower center:** Here the FRET efficiency histogram over the selected pixels will be plotted when the... ed.\\ - **Lower right:** Here the FRET distance histogram in terms of R0 will be plotted when the calculati... e FRET image is calculated, and the corresponding histograms are calculated. In the FRET image the FRET effic... o 100% FRET efficiency. * The FRET efficiency histogram and the Distances histogram are shown. {{ calcula
- calculate_ratiometric_single_pair_fret_distributions @howto
- y-step, how to calculate a [[glossary:fret|FRET]] histogram from single FRET pairs detected under single mole... .\\ - **Lower center/right:** FRET distribution histogram and FRET radius histogram. As these distributions have not been calculated yet, this area is still empty. ... s region is used to calculate the FRET efficiency histogram. The unmarked white area shows the fluorescence r
- lifetime_fitting_using_the_flim_analysis @howto
- ted.\\ **Upper right:** the Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... the lifetime graph(s) on the lower center (TCSPC histogram).\\ **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
- update @howto
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- roi_fitting_using_the_flim_script @howto
- ted.\\ - **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... the lifetime graph(s) on the lower center (TCSPC histogram).\\ - **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
- separation_of_2_species_with @howto
- e above highlighted in green. The photon counting histogram on the right displays the frequencies of the diff... are available, it is possible to load these TCSPC histograms as patterns for the filter definition. This is c... Matching" approach. * If the individual TCSPC histograms are not available, or a dye is analyzed that cha... ons, a lifetime fit can be performed of the TCPSC histogram of the measurement. The FLCS filters can be calcu
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- ale can be adapted.\\ **3: Fluorescence Lifetime Histogram**\\ Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\ **4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\ **5: [[glossary:TCSPC]] Decay window**\\ By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
- visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
- ted.\\ - **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... o the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\ - **Lower center/left:** [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red a
- data_file_import @howto
- ve TimeHarp260 software]]. This file contains two histograms: an [[howto:how_to_measure_the_instrument_respon... neral, such a ''.phu'' file may contain many more histograms. It is also possible that an IRF measurement an... container: {{ :howto:et2_workspace2.png |}} Two histograms have been imported. They are shown as "Decay" be... \ Unlike ''Decay_Coumarin_6.phu'' containing two histograms, both ''Anthracene_EtOH.phu'' and ''IRF375.phu''
- pre-histogrammed_image @glossary
- ====== Structure of the pre-histogrammed Image Data File====== ^Data Item ^^^^Type ^Descr... 'float32'' |time resolution of the TCSPC histograms in ns | |The following block will appe... r each ''t = 1 to <TCSPCChannels>''|||| | | | | ''HistogramData [x,y,t]'' |''int32''| counts of the TCSPC cha... For an example in Python and Matlab see here: https://github.com/PicoQuant/pre-histogrammed-Image-Data-File