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calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script

4 Hits, Last modified: 6 years ago

ratio of z to xy extension) is determined using a reference dye. This is the prerequisite to determine correc... "Absolute Diffusion Coefficients: Compilation of Reference Data for FCS Calibration" available from PicoQuan... Tutorial ===== ==== Select a file and generate a reference FCS curve ==== * Start [[products:SymPhoTime64... n constant is chosen as calibration value. If the reference were the concentration, the assignment can be cha

how_to_measure_the_instrument_response_function_irf

3 Hits, Last modified: 15 months ago

energy transfer (FRET)-based picosecond lifetime reference for instrument response evaluation \\ Measuremen... isional quenching of Erythrosine B as a potential reference dye for impulse response function evaluation\\ A... ichard D Ludescher, Ignacy Gryczynski\\ Standard reference for instrument response function in fluorescence

calculate_and_fit_fcs_traces_with_the_fcs_script

2 Hits, Last modified: 9 years ago

"Absolute Diffusion Coefficients: Compilation of Reference Data for FCS Calibration" available from PicoQuan... ur system regularly for correct alignment using a reference dye as ATTO488.\\ In this tutorial, we use the de

pattern_matching

2 Hits, Last modified: 11 years ago

e_23.png }} * In the pop-up window, select the reference-measurement (in the example select //''non-FRET s... Note:** This will import the overall decay of the reference measurement. Make sure the measurement contains o

antibunching_measurements

1 Hits, Last modified: 15 months ago

e see [[Diamond NV centers]] *depending on preference set laser repetition rate in order to allow full

using_the_antibunching_script

1 Hits, Last modified: 11 years ago

the photon pairs, detector 1 or 2 can be used as reference. The green curve is the average of both curves.