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- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- PhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRET ====== ... p-by-step, how to calculate a FRET histogram from single [[glossary:fret|FRET]]pairs detected under single molecule conditions using pulsed interleaved excitation... eptor dye.\\ The time traces can be acquired from single molecule events in a diluted solution, where the
- calculate_ratiometric_single_pair_fret_distributions
- PhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric Single Pair FRET Distributions ====== ===== Summary ===... calculate a [[glossary:fret|FRET]] histogram from single FRET pairs detected under single molecule conditions. The script requires a spectrally resolved fluoresce... eptor dye.\\ The time traces can be acquired from single molecule events in a diluted solution, where the
- flim-fret_calculation_for_single_exponential_donors
- e}} ~~NOTOC~~ ====== FLIM-FRET Calculation for Single Exponential Donors ====== ===== Summary ===== ... acceptor pair. If the FRET donor molecule has a single exponential decay kinetic, not only the FRET effi... fret|FRET]]. Several fluorescent proteins with a single exponential decay behavior have been reported, e.... Highlight the file ''FRET_GFP and mRFP.ptu'' by a single mouse click. {{ :howto:flim-fret-1expd_gret_gfp_a
- using_the_antibunching_script
- unching curve from a fluorescence time trace of a single emitter, in this example a nanodiamond NV center,... l are measurements of NV centers which are stable single photon sources excitable at 530 nm. The script is used to process one file with only a single NV center in the focus of the instrument. The oth... .\\ Antibunching measurements can be performed on single stationary molecules or at very low concentration
- flim_fret_calculation_for_multi_exponential_donors
- e file ''CENP-labelled _cells_for_FRET.ptu'' by a single mouse click. {{ flim-fret-calculation_for_multi-e... green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red an estimation... ''CENP-labelled _cells_for_FRET.ptu'' again by a single mouse click. {{ flim-fret-calculation_for_multi-e... can only be calculated if the FRET donor dye has single exponential decay kinetics, changing the fitting
- update
- ghlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse click. * Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... eparated regions with a similar lifetime into one single ROI, as it's done here, you have to press "Shift"... to:inner_monoexpo_3.png |}} ** Response:**\\ A single exponential reconvolution fit is performed. In th
- diamond_nv_centers
- s.png?250x180|Figure3: fluorescence spectrum of a single NV center in diamond excited with 532 nm}}] ====... oto-physical stability makes the nv-centers ideal single emitter samples compared to single molecules the laser power to excite them has to be considerably higher... *when measuring the fluorescence intensity of a single NV center, the count rate should stay stable. The
- lifetime-fitting_using_the_flim_script
- ghlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse click. * Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... oexpo_roi0_kidney_3.png |}} ** Response:**\\ A single exponential reconvolution fit is performed. In th... * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- * Highlight the file ''MicroBeads.ptu'' by a single mouse click. * Select the **"Analysis"** tab... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... { howto:monoexpo_roi0.png |}} **Response:**\\ A single exponential fit is performed. In the TCSPC window... ll as the IRF background and IRF Fit. * One the single pixel level, the background can be neglected, and
- visualizing_dynamics_using_the_multiframe-flim_script
- file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' by a single mouse click. * Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... it_multiframe_2.png?300 |}} ** Response:**\\ A single exponential reconvolution fit is performed. In th... * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
- lifetime_fitting_using_the_flim_analysis
- * Highlight the file DaisyPollen_cells_FLIM by a single mouse click. {{ lifetime-fitting_using_the_flim-s... green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red an estimation... it" (marked in orange). **Response:**\\ * A single exponential reconvolution fit is performed. In th... \\ In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
- symphotime_tips_and_tricks
- e [[products:MicroTime]], the image consists of a single frame) === FCS (for each of the max.2 data chan... cay is chosen as 0. Background pixels with just a single photon which might be before this pixel can there... * Every pixel in the image is exported as one single pixel in the bitmap * The color scale is not e... * Every pixel in the image is exported as one single pixel in the tiff * the tiff format for the nu
- antibunching_measurements
- ode]] *Start Point Measurement *For a single immobilized molecule antibunching trace you will ... u P., Ko H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/... B: //Ultrafast dynamics of protein collapse from single-molecule photon statistics//. In: //Proceedings o
- calculate_fccs_trace_with_the_grouped_fcs_script
- ight the file ''IBA488+547_crosslinked.ptu'' by a single mouse click. **Note:** This file contains a FCS ... lculate_fccs_Image_7.png }} * Press "Calculate Single". {{ calculate_fccs_Image_8.png }} **Response:*... negative calibration probe, i.e. a mixture of two single labeled compounds needs to be measured. To demons
- calculate_ratiometric_fret-images
- ns can only be separated using a donor dye with a single exponential decay kinetic and analyzed with lifet... This problem is too complex to be accessed with a single tutorial. In this tutorial, some arbitrary correc... e file ''CENP-labelled _cells_for_FRET.ptu'' by a single mouse click. {{ calculate_ratiometric_fret-images