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- intensity_time_trace_analysis
- lifetime_fitting_using_the_flim_analysis
- lifetime_fitting_using_the_tcpsc_fitting_script
- lifetime-fitting_using_the_flim_script
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- recording_a_fluorescence_lifetime_image_flim_stack_with_a_lsm_upgrade_kit_on_a_nikon_a1
- symphotime_tips_and_tricks
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- intensity_time_trace_analysis
- {{tag> tutorial howto time-trace analysis SymPhoTime}} ~~NOTOC~~ ======Intensity Time Trace Analysis====== ===== Summary ===== This... utorial shows step-by-step, how to draw intensity time traces from a single molecule. The script require... side of the main window. {{ using_the_intensity_time_trace_script_Image_1.png }} * Highlight the fi
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- {{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric ... cript requires a spectrally resolved fluorescence time trace, in which donor and acceptor have been exci... gJpZhrY?large }} **Note:** The script requires a time trace containing the fluorescence of two spectral... ainly the fluorescence of the acceptor dye.\\ The time traces can be acquired from single molecule event
- calculate_ratiometric_single_pair_fret_distributions
- {{tag> tutorial howto time-trace analysis FRET SymPhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric Sing... cript requires a spectrally resolved fluorescence time trace. As a result, the distribution of FRET effi... requisites ===== **Note:** The script requires a time trace containing the fluorescence of two spectral... ainly the fluorescence of the acceptor dye.\\ The time traces can be acquired from single molecule event
- avoid_pile_up_effect_in_flim_measurements
- the pile up limit the count rate displayed in the Time Trace below the FLIM image is used. * A red lin... is 10% of the laser repetition rate. * The **"Time Trace Binning"** should be set to 0.01 ms which e... for the Leica SP8 SMD. Here, the bin width in the time trace is fixed at 1 msec averaging many pixels. T... orrect average count rate.)) * At a pixel dwell time of 10µs each pixel is displayed as one point in t
- how_to_measure_the_instrument_response_function_irf
- ved. Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose. =... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
- how_to_work_with_the_instrument_response_function_irf
- ths of all contributions. The observed decay in a time domain measurement is a convolution of the 'real'... see this kind of curve: {{ howto:irf_decay_before_timegate.png?800 |}} (Before selecting a detection cha... pdated.\\ 4. Select the decay range by Setting a `Time Gate` (move the red bars) to limit the decay the ... **Note:** * The green area is used for the timegate. * In order to define the time range bette
- calculate_and_fit_fcs_traces_with_the_fcs_script
- page.\\ - **Upper center/right:** the intensity time trace. The display option can be changed using th... ing is not correlated with the laser pulse on the time - range of interest, photons caused by detector a... n various states with different photophysics at a timescale short or equal to the diffusion time through the confocal volume. Examples for these dyes are ATTO
- using_the_antibunching_script
- lculate an antibunching curve from a fluorescence time trace of a single emitter, in this example a nano... calculating an antibunching curve, a fluorescence time trace has to be recorded.\\ To separate the fluor... mber of simultaneously emitting molecules, a dead time free setup has to be employed for that time range. The SymPhoTime 64 script analyses data recorded in T
- flim_fret_calculation_for_multi_exponential_donors
- erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... lifetime corresponds to the real average arrival time of the photons and is therefore also close to the
- lifetime_fitting_using_the_flim_analysis
- erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... the decay in the TCSPC window. As a mean arrival time is calculated, it is possible that in dark region
- symphotime_tips_and_tricks
- easurement, the comment file can be edited at any time. The comment entered before the measurement is st... review display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on the type of analysis). Addit... alculated taking into account the pixel recording times) * Avg. Photons (in a pixel) * Avg. Countra... alculated taking into account the pixel recording times) * Frames (especially important for LSM upgra
- diamond_nv_centers
- NV center, the count rate should stay stable. The time trace in figure 8 shows a reduction of the countr... er choose a NV-center that is isolated, measuring time of 120 s should be enough to yield an antibunchin... Hz rep rate., 2304.94 a.u.;4 ms/pixel 45 min rec. time!}}] [{{ howto:diamond_roi_lifetime.png?270x300|... rate, Inhouse II, MPD/SPAD}}] [{{ howto:diamond_timetrace.png?270x200|Figure8: No bleaching visible. r
- lifetime-fitting_using_the_flim_script
- erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... pulse does not shift within the image acquisition time within the TCSPC window, therefore we fix the par
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... exponentials that have a lifetime longer than the time constant of the systematic errors. * Try last
- separation_of_2_species_with
- help page. - **Upper center/right:** Intensity time trace. The display can be changed using the "trac... n_of_2_species_with_Image_14.png?317 }} * This time, select the file ''ATTO655_diff_FLCS-pattern.ptu'... nt (cis/trans isomerization) at short correlation times is clearly visible. {{ separation_of_2_species_w... The photophysical component at short correlation times which was pronounced in the Cy5 correlation is n