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Fulltext results:
- syntax @wiki
- ==== Formatting Syntax ====== [[doku>DokuWiki]] supports some simple markup language, which tries to make the datafiles to be as readable as possible. Th... und:playground|playground]] page. The simpler markup is easily accessible via [[doku>toolbar|quickbutt... oo. ===== Basic Text Formatting ===== DokuWiki supports **bold**, //italic//, __underlined__ and ''m
- supported_mt200_pc_configuration_for_symphotime_32 @software
- ime MT200 MicroTime SPT32 PC}} ~~TOC~~ ====== Supported MT200 PC Configuration for SymPhoTime 32 ====== **NOTE:** When upgrading your system we strongly encourage you to d... sted PC from PicoQuant. While we try our best to support custom PC setups we cannot guarantee that all the components will work together. ===== Windows 1
- configuring_symphotime64_after_installation @support
- the user may be required when you want to: * upgrade SymPhoTime64 * change from SymPhoTime to ... 4 * to use SymPhoTime64 with a "home-made" set-up. ====Before Proceeding:==== These short instruct... hoTIme the user has to choose the exprimental set up type for which the SymPhoTime 64 will be used. \\ {{:support:spt64-install.png?300|}} -----------------
- software
- icoQuant takes no responsibility and provides no support. In case of problems please contact the respe... isting. Alternatively you can also use [[https://support.picoquant.com]] to contact PicoQuant support to submit your software project. ==== Data Analysi... orescence Spectroscopy and Imaging by research group Seidel, HHU]]: A collection of independent sotftw
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- es through the detection volume of a confocal set-up. Since then a series of studies have been perfor... living cells, including for example diffusion -coupled interactions like ligand binding, oligomerizat... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fluorescent molecules in a confocal setup the origin of the intensity fluctuations, will be
- supported_mt200_pc_configuration_for_symphotime_64 @software
- MadCityLabs Mad_City_Labs PC}} ~~TOC~~ ====== Supported MT200 PC and Hardware Configuration for SymPhoTime 64 ====== **NOTE:** When upgrading your system we strongly encourage you to d... sted PC from PicoQuant. While we try our best to support custom PC setups we cannot guarantee that all the components will work together. =====Supported
- flim_fret_calculation_for_multi_exponential_donors @howto
- pplication note [[http://www.picoquant.com/images/uploads/page/ files/7267/appnote_flim_fret.pdf]]. Th... The window contains five different regions:\\ **Upper left:** Imaging analysis options.\\ **Upper center:** Fast FLIM image, displayed in false color s... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
- avoid_pile_up_effect_in_flim_measurements @howto
- {{tag> howto FLIM acquisition pile-up TCSPC}} ~~TOC~~ ====== How to Avoid the Pile-up Effect in FLIM Measurements ====== For a definition of the pile-up effect see here: [[glossary:pile-up_effect|Pile-Up Effect]] To avoid [[glossary:pile-up_effect|pile-
- lifetime-fitting_using_the_flim_script @howto
- o tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}} ====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ====== ====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... **Response:**\\ FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
- separation_of_2_species_with @howto
- s <F1> to open the corresponding help page. - **Upper center/right:** Intensity time trace. The disp... esponse:** The FLCS pattern matching windows pops up. {{ separation_of_2_species_with_Image_8.png?600 ... ut the meaning of the different parameters. - **Upper center:** TCSPC histogram of the imported patt... n yet, this graph doesn't contain any data. - **Upper right:** TCPSC curve of the measured data. Low
- symphotime_tips_and_tricks @howto
- post-acquisition analysis is performed - speeds up the processing for additional analysis. - This ... times) * Frames (especially important for LSM upgrade kits where in general several frames are rec... with the MT200 this is done automatically, in LSM upgrade systems, which are not remotely controlled b... d "Pattern Matching" the feature is made availble upon clicking on the **"Resolution estimation"** tab
- pile-up_effect @glossary
- {{tag> TCSPC pile-up dead_time}} ~~TOC~~ ====== Pile-Up Effect ====== The Pile-Up effect describes the effects of photons lost at high photon count rates due... etect only one photon after one laser shot. "Pile up" describes the effects of photons lost at hight p
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
- o the tutorial [[Calculate FCCS Trace with the Grouped FCS Script]]. **Note:** This way you can compa... s <F1> to open the corresponding help page. - **Upper center/right:** Intensity time trace. The disp... esponse:** The FLCS pattern matching windows pops up. {{ using_the_flcs_script_for_spectral_crosstalk_... ut the meaning of the different parameters. - **Upper center:** The graph for the imported patterns.
- lifetime_fitting_using_the_flim_analysis @howto
- \ The window contains five different regions:\\ **Upper left:** imaging analysis options.\\ **Upper center:** Fast FLIM image, displayed in a false color ... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... e. In this case, the scale has to be adapted.\\ **Upper right:** the Fluorescence Lifetime Histogram.
- start
- wiki is a loose collection of documents that the Support team of PicoQuant found to be of general inte... Samples|Nitrogen Vacancy (NV) Centers]] ===== Support Documents ===== [[howto:start| HowTo's and Tutorial Collection]] [[software:supported_mt200_pc_configuration_for_symphotime_32|Supported MicroTime 200 PC Configuration for SymPhoTim