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Fulltext results:
- intensity_time_trace_analysis @howto
- {{tag> tutorial howto time-trace analysis SymPhoTime}} ~~NOTOC~~ ======Intensity Time Trace Analysis====== ===== Summary ===== This... utorial shows step-by-step, how to draw intensity time traces from a single molecule. The script require... side of the main window. {{ using_the_intensity_time_trace_script_Image_1.png }} * Highlight the fi
- fluorescence_correlation_spectroscopy-_a_short_introduction @general
- opulation of freely diffusing molecules. In short time, the theoritical framework of FCS was expanded to... udy of rotation diffusion. Unfortunately, at that time the application of FCS was impractical due to the... e mean product of the fluorescence intensity F at time t with the fluuorescence intensity after a lag time τ divided by the mean fluorescence intensity square
- software
- te software suite for acquisition and analysis of time resolved fluorescence data. SymPhoTime features a... EasyTau is the systems software of PicoQuants time resolved spectrometer [[products:FluoTime 300]]. ... ss communication and configuration with PicoQuant Time Correlated Single Photon Counting Instruments (TC... nce-Tools/tttrlib|tttrlib]] to read and correlate time-tagged time resolved (TTTR) files. * [[http:/
- t3-mode @glossary
- === T3-mode ====== (derived from [[tttr]]-mode or Time-Tagged-Time-Resolved mode) A special measurement mode of the [[products:PicoHarp 300]], [[products:Hyd... reduces the sync rate so that the channel [[dead time]] is no longer a problem. * The remaining prob... vent and the last sync event, and - the arrival time of the event pair on the overall experiment time
- create_time_gated_image
- l SymPhoTime}} ~~NOTOC~~ ====== How to create a time-gated Image in SymPhoTime ====== ===== Summary... torial shows step-by-step, how to create a simple time-gated intensity image in SymPhoTime ===== Step-b... , you'll find a green window with the header "Set Time Gate" {{:flim-image_after_loading.png|}} * Do... ate window, which can be amplified {{:flim-image_time-gate_selection.png|}} * Now a time gat can be
- some_origins_of_multiexponetial_decays_for_single_dyes
- arizers at magic angle, the rotation correlation time shows up as a second exponential in the decay (th... s in fact the product of the rotation correlation time and the fluorescence lifetime, divided by their s... riginal fluorescence lifetime, the observed decay time measured with a TCSPC spectrometer may be single-... o $\tau_A$ and $\tau_B$.// In this case the decay time measured in the spectral region of A would be sin
- t2-mode @glossary
- on about the channel it came from and the arrival time of the event with respect to the overall measurem... 4 bits for the channel number and 28 bits for the time-tag. Routing is not supported. If the time tag overflows, a special overflow marker record is inserted ... ssing of the data stream a theoretically infinite time span can be recovered at full resolution. [[Dead
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
- {{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric ... cript requires a spectrally resolved fluorescence time trace, in which donor and acceptor have been exci... gJpZhrY?large }} **Note:** The script requires a time trace containing the fluorescence of two spectral... ainly the fluorescence of the acceptor dye.\\ The time traces can be acquired from single molecule event
- calculate_ratiometric_single_pair_fret_distributions @howto
- {{tag> tutorial howto time-trace analysis FRET SymPhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric Sing... cript requires a spectrally resolved fluorescence time trace. As a result, the distribution of FRET effi... requisites ===== **Note:** The script requires a time trace containing the fluorescence of two spectral... ainly the fluorescence of the acceptor dye.\\ The time traces can be acquired from single molecule event
- avoid_pile_up_effect_in_flim_measurements @howto
- the pile up limit the count rate displayed in the Time Trace below the FLIM image is used. * A red lin... is 10% of the laser repetition rate. * The **"Time Trace Binning"** should be set to 0.01 ms which e... for the Leica SP8 SMD. Here, the bin width in the time trace is fixed at 1 msec averaging many pixels. T... orrect average count rate.)) * At a pixel dwell time of 10µs each pixel is displayed as one point in t
- how_to_measure_the_instrument_response_function_irf @howto
- ved. Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose. =... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
- calculate_and_fit_fcs_traces_with_the_fcs_script @howto
- page.\\ - **Upper center/right:** the intensity time trace. The display option can be changed using th... ing is not correlated with the laser pulse on the time - range of interest, photons caused by detector a... cs at a timescale short or equal to the diffusion time through the confocal volume. Examples for these d... It can be clearly seen that at short correlation time ranges, the curve does not fit the data well. Thi
- pile-up_effect @glossary
- {{tag> TCSPC pile-up dead_time}} ~~TOC~~ ====== Pile-Up Effect ====== The Pile-Up effect describes the ... s lost at high photon count rates due to the dead time of the TCSPC devices. Most single photon counti... detectors and [[TCSPC]] electronics have a [[dead time]]. After registering one photon the device needs some time to be ready for the next photon to detect. The de
- how_to_work_with_the_instrument_response_function_irf @howto
- ths of all contributions. The observed decay in a time domain measurement is a convolution of the 'real'... pdated.\\ 4. Select the decay range by Setting a `Time Gate` (move the red bars) to limit the decay the ... sed for the timegate. * In order to define the time range better, double click on the graph with the ... indow with you can enlarge in order to define the time gates better. When content, click o.k. * Deca
- using_the_antibunching_script @howto
- lculate an antibunching curve from a fluorescence time trace of a single emitter, in this example a nano... calculating an antibunching curve, a fluorescence time trace has to be recorded.\\ To separate the fluor... mber of simultaneously emitting molecules, a dead time free setup has to be employed for that time range. The SymPhoTime 64 script analyses data recorded in T