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- intensity_time_trace_analysis
- lifetime_fitting_using_the_flim_analysis
- lifetime_fitting_using_the_tcpsc_fitting_script
- lifetime-fitting_using_the_flim_script
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- recording_a_fluorescence_lifetime_image_flim_stack_with_a_lsm_upgrade_kit_on_a_nikon_a1
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- intensity_time_trace_analysis
- {{tag> tutorial howto time-trace analysis SymPhoTime}} ~~NOTOC~~ ======Intensity Time Trace Analysis====== ===== Summary ===== This... utorial shows step-by-step, how to draw intensity time traces from a single molecule. The script require... side of the main window. {{ using_the_intensity_time_trace_script_Image_1.png }} * Highlight the fi
- calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
- {{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric ... cript requires a spectrally resolved fluorescence time trace, in which donor and acceptor have been exci... gJpZhrY?large }} **Note:** The script requires a time trace containing the fluorescence of two spectral... ainly the fluorescence of the acceptor dye.\\ The time traces can be acquired from single molecule event
- calculate_ratiometric_single_pair_fret_distributions
- {{tag> tutorial howto time-trace analysis FRET SymPhoTime}} ~~NOTOC~~ ====== Calculate Ratiometric Sing... cript requires a spectrally resolved fluorescence time trace. As a result, the distribution of FRET effi... requisites ===== **Note:** The script requires a time trace containing the fluorescence of two spectral... ainly the fluorescence of the acceptor dye.\\ The time traces can be acquired from single molecule event
- avoid_pile_up_effect_in_flim_measurements
- the pile up limit the count rate displayed in the Time Trace below the FLIM image is used. * A red lin... is 10% of the laser repetition rate. * The **"Time Trace Binning"** should be set to 0.01 ms which e... for the Leica SP8 SMD. Here, the bin width in the time trace is fixed at 1 msec averaging many pixels. T... orrect average count rate.)) * At a pixel dwell time of 10µs each pixel is displayed as one point in t
- how_to_measure_the_instrument_response_function_irf
- ved. Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose. =... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
- calculate_and_fit_fcs_traces_with_the_fcs_script
- page.\\ - **Upper center/right:** the intensity time trace. The display option can be changed using th... ing is not correlated with the laser pulse on the time - range of interest, photons caused by detector a... cs at a timescale short or equal to the diffusion time through the confocal volume. Examples for these d... It can be clearly seen that at short correlation time ranges, the curve does not fit the data well. Thi
- how_to_work_with_the_instrument_response_function_irf
- ths of all contributions. The observed decay in a time domain measurement is a convolution of the 'real'... pdated.\\ 4. Select the decay range by Setting a `Time Gate` (move the red bars) to limit the decay the ... sed for the timegate. * In order to define the time range better, double click on the graph with the ... indow with you can enlarge in order to define the time gates better. When content, click o.k. * Deca
- using_the_antibunching_script
- lculate an antibunching curve from a fluorescence time trace of a single emitter, in this example a nano... calculating an antibunching curve, a fluorescence time trace has to be recorded.\\ To separate the fluor... mber of simultaneously emitting molecules, a dead time free setup has to be employed for that time range. The SymPhoTime 64 script analyses data recorded in T
- lifetime_fitting_using_the_flim_analysis
- etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... the decay in the TCSPC window. As a mean arrival time is calculated, it is possible that in dark region... the analysis. This also decreases the processing time. {{ lifetime-fitting_using_the_flim-script_Image
- flim_fret_calculation_for_multi_exponential_donors
- etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... lifetime corresponds to the real average arrival time of the photons and is therefore also close to the... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f
- diamond_nv_centers
- NV center, the count rate should stay stable. The time trace in figure 8 shows a reduction of the countr... er choose a NV-center that is isolated, measuring time of 120 s should be enough to yield an antibunchin... Hz rep rate., 2304.94 a.u.;4 ms/pixel 45 min rec. time!}}] [{{ howto:diamond_roi_lifetime.png?270x300|... : Antibunching measured on NV-center. Acquisition time 120 s, 1.3 N.A. oil immersion, 3534.98 a.u. 532 n
- flim-fret_calculation_for_single_exponential_donors
- erage "Fast FLIM" lifetime, i.e. the mean arrival time of the photons after the laser pulse. When not de... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... ated for each selected pixel, in which the longer time is kept constant and is considered as the donor l
- lifetime-fitting_using_the_flim_script
- etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... pulse does not shift within the image acquisition time within the TCSPC window, therefore we fix the par... e "Fast Lifetime", which is just the mean arrival time and contains e.g. background photons.\\ * An up
- lifetime-fitting_using_the_rapid_reconvolution_algorithm
- etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... exponentials that have a lifetime longer than the time constant of the systematic errors. * Try last ... the "fast Lifetime", based on the photon arrival time only. The Histogram on the right of the image con
- visualizing_dynamics_using_the_multiframe-flim_script
- age containing many frames is treated here like a time-series. The example image shows the plasma membra... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... ame binning of 10 in order to decrease processing time and increase photon numbers per pixel, while stil