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calculate_and_fit_fcs_traces_with_the_fcs_script
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ymPhoTime}} ~~NOTOC~~ ====== Calculate and Fit FCS Traces with the FCS Script ====== ===== Summary ===== This tutoria... ft side of the main window. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_1.png }} * Highlight the file ''A... '' by a single mouse click. {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_2.png }} * Select the "Analysis"-... en the dropdown menu "FCS". {{ calculate_and_fit_fcs_traces_with_the_fcs_script_Image_3.png }} **Note:** The drop-down me
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script
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measurements with a calibration dye. Therefore, a FCS curve is calculated with the FCS script, that is transferred to the FCS Calibration script. {{ youtube>K_dyVx-l_tY?large... d|SPAD]] or [[glossary:Hybrid PMT]] detectors).\\ FCS is a technique applicable over a wide range of concentrations, but ideal FCS concentrations are usually between 1 and 20 nM. =... ain window. {{ calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script_Image_1.png }} * Highlight ... ouse click. {{ calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script_Image_2.png }} * Select the
calculate_fccs_trace_with_the_grouped_fcs_script
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d in detail in the tutorial: "[[calculate_and_fit_fcs_traces_with_the_fcs_script|Calculate and Fit FCS Traces with the FCS Script]]". Look there to find out, how to proceed. ==== Need for calibration to r
separation_of_2_species_with
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ected in this graph. - **Lower center/right:** FCS trace window. As first the FCS correlation criteria have to be defined and the c... ng "Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:Calculate and Fit FCS Traces with the FCS Script]]. ==== Definition of... be fitted by selecting "Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:Calculate and Fit FCS Traces with the FCS Script]].
burst_duration_and_fcs_diffusion_time @howto:burst_duration_and_fcs_diffusion_time
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al howto analysis calibration PSF confocal-volume FCS burst single molecules measurement SMD Diffusion... duration}} ~~NOTOC~~ ====== Burst duration and FCS diffusion time ====== **Diffusion, flow, burst se... acted from fluorescence correlation spectroscopy (FCS) and the burst duration obtained from single-mole... -dimensional Gaussian detection model ===== Both FCS and burst-based single-molecule experiments rely
using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs
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tified in this graph. - **Lower center/right:** FCS trace. As first the FCS correlation criteria have to be defined and the c... al FIT" to fit these patterns to the decay of the FCS measurement. {{ using_the_flcs_script_for_spectr... nlinked_mix.ptu'') is active. ==== Calculate the FCS crosscorrelation curve ==== * Press "Cross Cor... f the FCCS correlation obtained with the "Grouped FCS" script to better see the effect. {{ using_the_fl
symphotime_tips_and_tricks
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the script with the stored result - For online FCS-results, the calculated molecular brightness can ... result-file opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on ... me]], the image consists of a single frame) === FCS (for each of the max.2 data channels) === <WRAP> <imgcaption CommentFCS| Comment Tab of an FCS online analysis result>{{onlinefcs-3.png?300|}}</
2ffcs
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fusion length into the analysis. see [[glossary:FCS]] for a introduction on FCS. ===== Experimental Setup ===== ==== Pinhole Sel... i === The distance between the foci in two-focus FCS is determined by the Nomarski prism. Usually the
performing_an_fcs_measurement_with_an_olympus_fv1200_upgrade_kit
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{{tag> howto FCS acquisition video FV1200 Olympus LSM_upgrade}} ====== Performing a FCS measurement with an Olympus FV1200 Upgrade Kit===... } This video is a walkthrough of how to measure a FCS (Fluorescence Correlation Spectroscopy) trace with an Olympus FV1200 upgraded for FLIM and FCS with a PicoQuant upgrade kit. Continue to: {{t
howto
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). ==== General ==== {{topic>tutorial +analysis -FCS -FRET -FLIM -draft &nodate&nouser}} ==== FLIM ==== {{topic>tutorial +analysis +FLIM -FCS -FRET -draft &nodate&nouser}} ==== FRET ==== {{t... al +analysis +FRET -draft &nodate&nouser}} ==== FCS / Correlation ==== {{topic>tutorial +analysis +FCS -draft &nodate&nouser}} ===== EasyTau2 / Analysis
flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade
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FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade ====== This tutorial shows the recording... NIS Elements 4.3 integrates control for FLIM and FCS ===== {{ youtube>d_-4MSWznkU?large }} The movie... Kit on a Nikon A1 ]] * [[FLIM-FRET Measurement using an Olympus FV1200 with a FLIM and FCS upgrade]]
phasor_analysis
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9%20-%20Reading%20PicoQuant%20PT3%20files%20for%20FCS%20and%20lifetime%20FCS.pdf| Reading PicoQuant PT3 files for FCS and lifetime FCS]] Additional Tutorials for the Globals software can be found here: [[http://www.lfd
mt200fcs
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======MT200 FCS Measurement ====== {{tag>howto MicroTime200 video tutorial}} fluorescence correlation spectroscopy (FCS) is a quantitative measurement of the resolution
phasor_plot_structure_separation @howto:phasor_plot_structure_separation
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NIS-Elements version 6.10.01 with PicoQuant FLIM-FCS full integration {{::nis_phasor_publish.mp4... NIS-Elements version 5.42.06 with PicoQuant FLIM-FCS full integration {{:lifetime_component_deco
calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
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are unsure which binning to select, calculate the FCS curve and check the diffusion time to see the ave
intensity_time_trace_analysis
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antibunching_measurements
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using_the_antibunching_script
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calculate_ratiometric_single_pair_fret_distributions
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flim-fret_measurement_using_an_olympus_fv1200_with_a_flim_and_fcs_upgrade
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determination_of_the_focal_width_with_the_focal
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