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Fulltext results:
- flim_fret_calculation_for_multi_exponential_donors @howto
- {{tag>tutorial howto analysis FLIM imaging FRET SymPhoTime}} ~~NOTOC~~ ====== FLIM-FRET Calculation fo... _for_FRET.ptu''. Thereby, a new Window opens: {{ :howto:flim-fret-multiexpd_analysis_flim.png |}} **Note... channel the donor emission has been recorded. {{ :howto:flim-fret-multiexpd_ch2.png?400 |}} * Press "C... transfected only with the donor constructs. {{ :howto:flim-fret-multiexpd_enahncedcontrast.png?400 |}}
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- {{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}} ====== Lifetime-Fi... art the FLIM analysis by clicking on "Start". {{ howto:flimgui.png?200 |}} **Response:**\\ The FLIM an... o the data file. Thereby, a new Window opens: {{ howto:sptwindows_rapidflim.png |}} **Note:**\\ The w... etime range for histogram on the right hand. {{ howto:contrastadaptation2.png?600 |}} * Set Proper
- applications
- ications ===== ==== Fluorescence Lifetime ==== [[howto:lifetime_fitting_using_the_tcpsc_fitting_script| ... video_tutorials| FLIM by Philippe Bastiaens]] [[howto:how_to_measure_the_instrument_response_function_i... asure the Instrument Response function (IRF)]] [[howto:lifetime_fitting_using_the_flim_analysis|Lifetime fitting Using the FLIM analysis software]] [[howto:roi_fitting_using_the_flim_script|ROI fitting usi
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- {{tag>howto FLIM multi-frame imaging analysis dynamics tutorial LSM_upgrade SymPhoTime}} ====== Visualiz... rame FLIM" analysis by clicking on "Start".\\ {{ howto:mflimgui.png?300 |}} **Response:**\\ The FLIM... DCK_Cells.ptu''. Thereby, a new Window opens: {{ howto:fullscreen_tensionsensor_2.png |}} **Note:**\\ T... lation by clicking on "Calculate FastFLIM".\\ {{ howto:framebin_10_3.png?300 |}} **Response:**\\ An ima
- update @howto
- {{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}} ====== ROI Fitting Using the FLIM Analys... rt the FLIM script by clicking on "Start".\\ {{ howto:startflim.png?200 |}} **Response:**\\ The FL... lls_FLIM.ptu''. Thereby, a new Window opens: {{ howto:kidneyfullscreen3.png |}} **Note:**\\ The windo... channel 4 - and press "Calculate FastFLIM".\\ {{ howto:detectionchannel.png?400 |}} **Response:**\\ Th
- flim-fret_calculation_for_single_exponential_donors @howto
- {{tag>tutorial howto FRET FLIM imaging analysis SymPhoTime}} ~~NOTOC~~ ====== FLIM-FRET Calculation fo... ce panel on the left side of the main window. {{ :howto:flim-fret-1expd_samples_ws.png |}} * Highlight... T_GFP and mRFP.ptu'' by a single mouse click. {{ :howto:flim-fret-1expd_gret_gfp_and_mrfp.png |}} * Se... in there, open the drop down menu "Imaging". {{ :howto:flim-fret-1expd_analysis_imaging.png?500 |}} **N
- lifetime-fitting_using_the_flim_script @howto
- {{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}} ====== Lifetime-Fitting Using the ... e FLIM analysis. Thereby, a new Window opens: {{ howto:kidneyfullscreen3.png |}} **Note:**\\ The window... I" checkbox and click "ROI from threshold".\\ {{ howto:activateroi.png |}} **Response:**\\ A large win... a threshold for an image analysis visually. {{ howto:selectthreshold_kidney3.png |}} **Note:**\\ Lef
- measuring_quantum_yield @howto
- g> FT300 easyTau Quantum_Yield Integrating_Sphere howto tutorial}} ====== Measuring the Quantum Yield wi... an indexed lid to ensure proper orientation. {{ :howto:2013-12-19_at_14-30-03.jpg?600 |}} * Use the p... he sample holder into the integrating sphere {{ :howto:2013-12-19_at_14-35-37.jpg?600 |}} * Select ei... ight originating from the sphere's surface). {{ :howto:2013-12-19_at_14-35-27.jpg?600 |}} * Switch on
- measurement_hardware_instrumentation
- me 300#Time Resolved Emission Spectra (TRES)]] [[howto:measuring_quantum_yield|Measuring the Quantum Yie... oftware:EasyTau#Scripts | EasyTau Scripts]] /*[[howto:how_to_measure_the_instrument_response_function_i... Kurt Thorn]] [[Beampath of the Zeiss LSM880]] [[howto:check_overlap_of_different_color_confocal_volumes... overlap of different color confocal volumes]] [[howto:determination_of_the_focal_width_with_the_focal|D
- diamond_nv_centers @howto
- {{tag>howto antibunching correlation sample}} ~~TOC~~ ====== Diamond NV Centers ====== =====Basics===== see also [[wp>Nitrogen-vacancy center]] [{{ howto:model_nv-centers.png|Figure1: Model of the nitrog... ox. 12ns. ===== Measuring NV centers ===== [{{ howto:jablonski_nv-centers.png|Figure2: Jablonski diagr... ors) *objective: 1.3 N.A. oil immersion [{{ howto:emission_nv-centers.png?250x180|Figure3: fluoresc
- data_file_import @howto
- ate a new workspace or open an existing one: {{ :howto:et2_createopen.png | File menu }} Then select I... alog opens, where the data file is selected: {{ :howto:et2_fileimport.png | Selects the file to be impor... ftware]]. This file contains two histograms: an [[howto:how_to_measure_the_instrument_response_function_i... ned in the native software, looks like this: {{ :howto:et2_originalhistograms.png | Original data file c
- how_to_work_with_the_instrument_response_function_irf @howto
- {{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}} ====== How to Work with Instrument Response F... ator the timing resolution of the instrument. {{ howto:irf.png?500 |}} The IRF width is approx. a quad... re described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_response_function_i... ral strategies are possible which are described [[howto:how_to_measure_the_instrument_response_function_i
- symphotime_tips_and_tricks @howto
- {{tag>Howto Analysis Tutorial SymPhoTime Tips&Tricks}} ====== SymPhoTime 64 Analysis Tips and Tricks... ice direction"** in the following GUIs \\ \\ {{:howto:slice_direction.jpg?250|}} \\ \\ * FLIM * ... icking on the **"Resolution estimation"** tab. {{:howto:resestimationflim.jpg?250|}} \\ \\ or \\ \\ {{:howto:pattern_resolution_est.jpg?250|}} Let us take a
- howto @howto
- ====== HowTo's and Tutorials ====== ===== Instrumentation ===== ==== Fluorometer ==== {{topic>howto support +FT300 -sample -analysis -draft &nodate&nouser}} ==== Microscopy ==== {{topic>howto -FT300 -sample -analysis -draft &nodate&nouser}} ... .etc'' files can be opened directly, using the {{:howto:ff.png|}} button. * It is also possible to use
- reconvolution_fit @howto
- cay curve. Open the Fitting Window by pressing {{:howto:ff.png?40|}}. {{ :howto:et_sel1.png |}} \\ \\ The last curve in the container, ''crv[1]'', a decay c... an IRF and then change the fitting modality. {{ :howto:et_fitwindow1.png |}} \\ \\ Clicking on (select... e type" can be changed from "Decay" to "IRF". {{ :howto:et_fitwindow2.png |}} \\ \\ The plot is updat